ع ذ ثح س ا ذسخ ا راث ١ خ ا زى خ ١ ب ا ح ٠ ١ خ خب عخ ذ ٠ خ ا سبداد د./ مرطفي عبد الرمد مرطفي صقر استاذ اله ارثة و التذخيص الجزيئي - قدم المذخرات الجزيئ

النسخ

1 ع ذ ثح س ا ذسخ ا راث ١ خ ا زى خ ١ ب ا ح ٠ ١ خ خب عخ ذ ٠ خ ا سبداد د./ مرطفي عبد الرمد مرطفي صقر استاذ اله ارثة و التذخيص الجزيئي - قدم المذخرات الجزيئية معهد الهندسة اله ارثية- جامعة مدينة الدادات اإلسم )رباعى(: مرطفي عبد الرمد مرطفي صقر الجهة التابع لها: جامعة مدينة الدادات المعهد معهد بحهث الهندسة اله ارثية والتكنهلهجيا الحيهية 4224/2/42 تاريخ التعيين : الحالة اإلجتماعية: متزوج + 3 القدم العممى: قدم المذخرات الجزيئية الدرجة العممية : دكتهر اه الفمدفة فى بيهلهجيا الدرطان الجزيئية الهظيفة الحالية: استاذ اله ارثة و التذخيص الجزيئي التخرص العام: طب بيطري- الهندسة اله ارثية والتكنهلهجيا الحيهية التخرص الدقيق: التذخيص الجزيئي لالم ارض العمل: المهبايل: التميفهن : فاكس: Mostafa.sakr@gebri.usc.edu.eg dr_sakr2000@yahoo.com البريد اإللكترونى:

2 العنهان: معهد الهندسة اله ارثية- المنطقة الخامدة- مجمع اله از ارت- مدينة الدادات- محافعة المنهفية - 1 ثكبن ز ض ف انعه و انطج خ انج طس خ عبو جبيعخ غ طب كه خ انطت انج طسي ثكفسانش خ يع د اثحبس انعسغب - جبيعخ ف عهى ث ن ج ب انعسغب انجص ئ خ عبو دكز زا انفهعفخ كب بشا - ان بثب - يدددزض ثمعددى ان ش صددبد انجص ئ ددخ ث ع ددد ثحدد س ان دظددخ ان زاث ددخ انزك ن ج ددب انح ددخ- جبيعدد خ يد ددخ انعبداد ي ر يبزض 2202 حزى ا. - يدزض ثمعى ان ش صبد انجص ئ خ ث ع د ثح س ان دظخ ان زاث خ انزك ن ج ب انح خ- جبيع خ ان ف خ ي ر ب س 2200 ان يبزض يع د ث ع د ثح س ان دظخ ان زاث خ انزك ن ج ب انح خ- جبيعخ ان ف خ انزش ص انجص ئ ناليساض ان عد خ ان زاث خ انز رص ت اال عب انح ا ثبظدز داو انطدسا انحد دخ فد انج ن ج ب انجص ئ خ - دزاظبد زاث خ جص ئ خ عه ثعط انج بد انز ن ب ربث س عه حدد س كز دس يد االيدساض ي دب ان زاث دخ ا االيساض انعسغب خ - اال ز بو ثدزاظخ انج ن ج ب انجص ئ خ نه ال ب انعسغب خ - عالقخ ا ص ى MT1-MMP ثحد س, رط ز ا زمبل ان ال ب انعسغب خ -2 ث ١ ب ثب مرراد ا ذراس ١ خ ا ز شبرن ف رذر ٠ س ب 0 -ث ن ج ب انعسغب 2 -رحبن م ي بع خ أجعبو ي بع خ غسا ر ب ل اندي ا ا 2

3 6 -ان ال ب انه فب خ 8- انك بء انح خ نالح بض االي خ - 2 اجعب ي بع خ عهى ان بعخ انك بئ ان ه ي 5- انف س ظبد انطج خ 7- عهى ان بعخ انك بئ ان ه ي 9- غسا رطج مبد اظ بد ان بدح ان زاث خ ث ١ ب ثب اد ا ز لب ثز ص ١ ف ب 0- ث ن ج ب انعسغب 2- رحبن م ي بع خ ان شبزكخ ف رج ص يع م قعى ان ش صبد انجص ئ خ ذنك ثبن شبزكخ ف ظع كزبثخ ي اصفبد األج صح ان شبزكخ ف نجب انجذ انف ان بن نشساء األج صح انك ب بد رشغ م ان ع م. ان شبزكخ ف ر خ ي بزاد ان ئخ ان عب خ ثبنمعى رمد ى ان صح انز ج إلجساء انزجبزة انع ه خ ف يع م انمعى. ان شبزكخ ف أع بل ر ظعخ ظى يعبيم إظبف خ نهمعى اظزحداس يجبالد ثح خ يسرجطخ ثبنز صصبد اندق مخ نهمعى ي اكجخ نه عزحدثبد ف يجبل انزش ص انجص ئ انعالج بد. عع خ انهجب انف خ ان ج مخ ع يجهط ان ع د نج خ انحبظجبد ) ( نج خ ان زجساد االج صح انعه خ ) (. ان شبزكخ ف نجب انجذ انف ان بن ان بصخ ث بقصبد شساء ك ب بد ر ز د ب ان ان ع د. عع خ انفس ق انز ف ري ن حدح رم ى األداء ر ك د انج دح ثبن ع د يع ل نج خ ان ساجعخ االكبد خ نهجسايج عع خ انفس ق انز ف دي نه ع م ان سكصي نهزك ن ج ب انح خ ان شسف عه ج بش HPLC نعبو ج بش RT-PCR ف انفزسح ي ان اال. ان شدبزكخ فد يحبظدساد دد اد ان شدبغ انعه د نمعدى ان ش صدبد انجص ئ دخ حعد ز ي بقشدبد انسظدبئم انعه دخ ثبنمعى ثبق أقعبو ان ع د. 2

4 ان شبزكخ ف إجساء اخزجبزاد انمج ل نهطهجخ ان زمدي نهزعج م ثبندزاظبد انعه ب ثبنمعى ) ( عع فى انج ع خ ان صس خ نهعه و انج ن ج ب إثزداءا ي 2205 حزى ا Workshop about the "Bioanalyzer" held on Genetic Engineering and Biotechnology Research Institute, Sadat City, Minoufiya University, Egypt. 21 st November 2012 Workshop in the module entitled: (Proposal Writing Post Docs). Administrated by the DAAD Cairo held at Faculty of Veterinary Medicine, Sadat City, Minoufiya University, Egypt. 2 nd December Workshop and training course on (HPLC 1260) Adminstrated by Agilent Technologies Company held on Genetic Engineering and Biotechnology Research Institute, Sadat City, Minoufiya University, Egypt. 19 th December Trainee in the 2-day training workshop about PCR technique held in the Molecular Diagnostics andtheraputics Department, GEBRI, University of Sadat City from October Cairo Initiative (USAID) Alumni Workshop held on October 20th 2014 at Golden Tulip Hotel Flamenco located in 2 El Gezira El Wosta Street - Zamalek. Egypt Workshop in "Ethical guidelines for Animal Care and Use in Scientific Reseaerch" organized by Institutional Animal Care & Use committee, Faculty of Veterinary Medicine, Sadat City held at Faculty of Veterinary Medicine, University of Sadat City, Egypt. 28 th November Workshop on " Nanotechnology and Applications" organized by Genetic Engineering and Biotechnology Research Institute (GEBRI) in cooperation with Naqaa Foundation of sceientific Reseaerch, Technology and Development, held at GEBRI, University of Sadat City, December

5 Moustafa A. Sakr and Hiroshi Sato. The role of membrane type 1- matrix metalloproteinase (MT1-MMP) in cancer invasion and metastasis. Attendance and oral presentation in the first Egypt Japan international Symposium of science and technology, June 8-10, Japan Attendance International symposium on tumor biology in Kanazawa, January 2007, Japan. Attendance International symposium on tumor biology in Kanazawa, January 2008, Japan. Attendance International symposium on tumor biology in Kanazawa, January 2009, Japan. Moustafa A. Sakr and Hiroshi Sato. The interaction between GI24 and Membrane tupe1-matrix metalloproteinase (MT1-MMP) in cancer invasion and Metastasis. Attendence and poster presentation in the joint symposium of 5 th international symposium of institute network in Kanazawa June Japan. Attendance 19th Annual meeting of Japanese association for metastasis research. 17th June 2010, Japan. Attendance in International symposium on tumor biology in Kanazawa, cancer and host response, 14 th international congress on immunology satellite symposium th August 2010, Japan. Oral presentation about the scientific mission awrded by USAID in the GEBRI on 20 July 2014 titled by "MT1-MMP in cancer and tissue development" Giving a lecture titled by "how to study the gene function (molecular approach" 24 September Attendence in "International Symposium in Tumor Biology in Kanazawa. 8 April 2015, Takara hall, Kanazawa University hospital, Japan. Attendance in the "1 st Anuual National Conference for Expremintal Research". 5 th September 2015, children hospital Mansoura University. Attendance "The first Edition of Egyptian Biotech Joiners" organized by "Bio team Egypt" in collaboration with NRC on 29 th October Moustafa Sakr (2016). mir-150-5p and mir-133a inhibit glioma cell proliferation and migration by targeting MMP14. Attendance and sharing with 5

6 abstract (oral presentation) in the 1 st International Conference of Genetic Engineering and Biotechnology "Intergrative Science and Biotechnolohy" sharm El-sheikh April Organized by GEBRI, University of Sadat City. - ان شبزكخ ك د س ف يشبزك ف يشس ع يع م ان ش صبد انجص ئ خ انعالجبد انج يبد ان ل يشبزكخ ي جبيعخ يد خ انعبداد ادازح ان شس عبد ف شازح انزعه ى انعبن رحذ ك د LP9-031-SDAT زع اظز داو انج م ان ب ي اج صح يعسفخ رزبثعبد اندي ا أ را ان شس ع ي اال خ ث كب ن ب ن ي ظسعخ ف رحد د رزبثعبد ان بدح ان زاث خ رحد د انطفساد ف انج بد ان سظخ ان عججخ ناليساض ان زاث خ كرنك ايبك خ ع م يعح نهج و انجشسي ثبظز داو را انج بش. اس ا طب ت ع ا ا رسب خ ا زم ١١ ا دس ٠ ئ ي COX-2 P53 سرغب ا ىجذ ف ا درزا ا ثزحثخ ث بدح ا ذا ا ٠ ث ١ ١ زر زا ١ ظذ ا ذالالد ا س ١ د ١ خ ا رظ ١ خ ا م ١ خ ا زشخ ١ ص ١ خ ١ ىر ر ب" 615-5p " ف ا ىشف ا جىر ع سرغب ا ىجذ ا صر ١٠ رم ١١ د ر ا ١ ىر ار ا ا 21-٠ ف ر ظ ١ ا زعج ١ ر ا د ١ د ١ ا ثجػ ر ث ١ ز ف رظ سرغب ا ىجذ ا ذ ر ا زشخ ١ ص ا زعج ١ ر ا سائذ جر ر ١ ا ١ زب ثر ر ١ ١ س 12 ف رظ سرغب ا ىجذ ا زم ١١ ا دس ٠ ئ بدح ا س ١ ز و ١ رار ١-1 ف رشخ ١ ص رض سرغب ا ىجذ ا برح ع االصبثخ ثف ١ ر ش ش ا ذ ر ا زشخ ١ ص ا ز جئ بدح ا د ١ ج ١ ىب -3 ف رظ سرغب ا ىجذ ا ذرخخ ا ع ١ خ دوز را دوز راح دوز راح بخس ١ ر بخس ١ ر د خ االشراف أ.د/ ا بي اح ذ عجذ ا عس ٠ س أ.د/ دمحم ا شحبد عج ١ ذ أ.د/ ح د ا ب صر أ.د/ شب عجذ ا صبدق اس بع ١ أ.د/ شب عجذ ا صبدق اس بع ١ د/ دمحم ٠ سر عجذ ا ىر ٠ د/ صطف عجذ ا ص ذ صطف د/ دمحم ٠ سر عجذ ا ىر ٠ د/ صطف عجذ ا ص ذ صطف د/ دمحم ٠ سر عجذ ا ىر ٠ د/ صطف عجذ ا ص ذ صطف 1 - ح د شم ١ ر 2- ف ١ ذح عجذ ا سال وشه 3-١ ذ ف ا ع ب 4 - ج دمحم ح د 5- بء دمحم اح ذ دمحم 6 -سحر ا س ١ ذ دمحم ع 6

7 7 -ا ١ رح عجذ هللا عجذ ا رح 8 -ا ١ رح عجذ ا عس ٠ س 9 -حس ا رهللا دمحم 10 -حبز اث ١ خ ا برح ع االصبثخ ثف ١ ر ش ش ا زم ١١ ا دس ٠ ئ بدح HGF وذال خ ع حذ س سرغب ا ىجذ ف اال سب ا زم ١١ ا دس ٠ ئ بدح MDK ف رشخ ١ ص رظ سرغب ا ىجذ ا زم ١١ ا دس ٠ ئ بدح GGT وذال خ ع حذ س سرغب ا ىجذ ف اال سب ا زم ١ ا دس ٠ ئ ي ADAM8 وذال خ ع حذ س سرغب ا ىجذ ف اال سب رعذد االشىبي ا رف خ ١ خ د ١ بد MTHFR ACE ا زع مخ ثبالسزدبثخ ا ا عالج ا خبفط عغػ ا ذ ا ررفع ا صر ٠ رعذد االشىبي ا رف خ ١ خ د ١ بد CYP2A ACEا زع مخ ثبالسزدبثخ ا ا عالج ا خبفط عغػ ا ذ ا ررفع ا صر ٠ ا زع جر ا د ١ PRAME CD95 رد د االو ١ ١ ى رظ سرغب ا ذ ا حبد ث ١ ا صر ١٠ رعذد االشىبي ا رف خ ١ خ د ١ بد K-ras PTEN ا زع مخ ثب راض سرغب ا رح ف ا رظ ا س ١ ذاد ا صر ٠ بد ا م ١ خ ا زشخ ١ ص ١ خ حب ط ا ١ سف سفبرذن (LPA) ثب مبر خ اال فبف ١ ز ثر ر ١ (AFP-L3) ا جر ثر ج ١ ا دسا ا برح ع مص ف ١ زب ١ (PIVA) K ف سرغب ا ىجذ ا زم ١١ ا دس ٠ ئ د ١ بد EpCAM FGFR3 و س ١ خ جىرح زشخ ١ ص سرغب ا ثب خ ف اال سب ا زشخ ١ ص ا بع ا دس ٠ ئ ح Q بخس ١ ر ا.د/ شب عجذ ا صبدق ا.د/ اح ذ شعرا ا.د/ شب عجذ ا صبدق ا.د/ اح ذ شعرا ا.د/ شب عجذ ا صبدق ا.د/ اح ذ شعرا ا.د / دمحم فزح فرج ث ١ ا.د / شب عجذ ا صبدق اس بع ١ د/ صطف عجذ ا ص ذ صطف ا.د / شب عجذ ا صبدق د/ صطف عجذ ا ص ذ صطف أ.د/ دمحم عث ب عجذ ا فزبذ 11- اح ذ ع بد شف ١ ك دمحم 12- اح ذ رزق عجذ ا بة 13- ا ٠ بش ح 14 -د ٠ ب ع رشبد ا دسار د/ دمحم عجذ ا رح ١ دمحم س ١ ب أ.د/ دمحم عث ب عجذ ا فزبذ أ.د/ دمحم عث ب عجذ ا فزبذ 15- ا ٠ ز حدبز 16- بي عص ذ صطف 17- دمحم صجح غ ١ ا زشخ ١ ص ا بع ا دس ٠ ئ ف ١ ر ش ح ا ١ ا غرث ا زط ر ا دس ٠ ئ ا ى ١ ١ بئ ا ح ١ ي 67-k htert ا جر ر ١ p53 ف أ.د/ صجبذ فبر ق ا عجذ أ.د/ صجبذ فبر ق ا عجذ أ.د/ ا بي اح ذ عجذ ا عس ٠ س 18- ح ذ ع عجذ ا عس ٠ س عجذا سال 19- عجذ ا بة عجذ ا حس شرف 7

8 20- دمحم ص ر عجذ ا ح ١ ذ خبدهللا مب خ األد ٠ خ ا زعذدح عالج سرغب ا ىجذ ا ١ ىر أر أ أ و ؤشر ح ١ زشخ ١ ص ا جىر سرغب ا ثذ أ.د/ را ذا دمحم غ عذ قائمة بمجمل األبحاث والممخرات 1. Sakr MA, Takino T, Domoto T, Nakano H, Wong RW, Sasaki M, Nakanuma Y, Sato H. GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. Cancer Sci. 2010, 10: AboElkhair M, Bazid AI, Sakr MA, Abd El-Razak AG, Sultan H (2012). Molecular characterization of fusion protein of Newcastle disease virus in Egypt (2012). Egyptian J Virol 9: ISSN: Sakr MA., Gado MS and Elokil AA (2014). Molecular isolation, cloning and sequencing of chicken insulin-like growth factor 1 (IGF-1) gene from high producing exotic breed to produce transgenic chickens of native breeds in Egypt. 2 nd International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada, 8-12, April 2014, published as special issue on Annals of Agriculture Science, Moshtohor. ISSN: Isamail HA, Elsaid HH, Elfert AY, Sakr MA, Alnoamany WF. Evaluation of the Role of MicroRNA-21 in Regulating the Expression of Phosphatase and Tensin Homolog (PTEN) in Egyptian Hepatocellular Carcinoma Patients. Menoufia Medical Journal, 2015 (in press) Tarabees1 RZ, Hassanin ZH, Sakr MA, Zidan SM (2016). Molecular Screening of Some Virulence Factors Associated With Staphylococcus Aureus Isolated From Some Meat Products. Alex J. veterinary science, 48(1): ISSN: DOI: /ajvs Sakr M, Takino T, Sabit H, Nakada M, Li Z, and Sato H (2016). mir-150-5p and mir-133a suppress glioma cell proliferation and migration through targeting 8

9 membrane-type-1 matrix metalloproteinase. Gene; 587 (2016) : Raiuf AA, Hamdy MM, Shalaan OF, Sakr MA, Abdel Rahman AA (2016). MTHFR A1298C polymorphism and the Vaso-Occlusive crisis in sickle cell disease. 1 st international conference of genetic engineering and biotechnology "Intergrative Science and Biotechnology" Sharm El-sheikh April Published as special issue in Research Journal of Applied Biotechnology (RJAB) ISSN: Shafiq A, Sakr M, Hemieda H, Elnasr M, Ismail H (2016). Association of MTHFR (C677T) and ACE (I/D) polymorphisms with hypertension and response to treatment among Egyptian. Egyptian J. of genetics and cytology (2016) 45(1): ISSN: X Shalaan O, Sakr M, Daif A, Elhalfawy K (2016). Rapid and simultaneous detection of β- thalassemia point mutations by reverse hybridization StripAssay among Egyptian patients. Journal of Biochemistry, Microbiology, and Biotechnology (in press) vol. 4(1): ISSN:

10 Curriculum Vitae Personal information: Name: Moustafa Abdel Samed Moustafa Sakr Date of birth: 13 th October 1975 Place of birth: Kafr El-Shaekh Nationality: Egyptian Marital status: Married+ 2 Contact address: Molecular diagnostics department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt Telephone no: Fax no : mostafa.sakr@gebri.usc.edu.eg, dr_sakr2000@yahoo.com Education: B.V.Sc., Fac.Vet.Med. Tanta University, Egypt, Doctoral of Philosophy (Ph.D) in molecular tumor biology, Cancer Research Institute, Kanazawa University, japan, Thesis title: Expression cloning of molecules that interact with membrane type 1- matrix metalloproteinase (MT1-MMP) and analysis of contribution of GI24 to MT1-MMP-medaited tumor invasion. Professional Positions 2002 till now Researcher in field of molecular diagnosis (Genetic engineering and Biotechnology Research Institute, Egypt) master fellowship in Molecular diagnostics department (Genetic engineering and Biotechnology Research Institute, Egypt) : Doctoral fellowship in Molecular Virology and Oncology department, Cancer Research Institute, Kanazawa University, Japan. November May 2014 Postdoctoral fellowship for 6 months at Division of Molecular Medicine and Genetics, Department of Internal Medicine, The Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA. Weiss Lab. 02

11 December June 2015 Postdoctoral fellowship for 6 months at Molecular Virology and Oncology department, Cancer Research Institute, Kanazawa University, Japan. Research interest: Molecular diagnosis of infectious and genetic diseases. Matrix metalloproteinases field (especially MT1-MMP) their substrates and their roles in cancer invasion and metastasis. Field of specialization: Genetic engineering and Biotechnology Research (Molecular diagnosis field) Matrix metalloproteinase type 1- metalloproteinase (MT1-MMP) and cancer invasion and metastasis Languages: Arabic: Mother tongue English: Good command Computer Skills: Touch typing, good user of Microsoft Office programs and Internet applications Practical Research experiences 1- Basic molecular biology techniques: - Construction and screening of plasmid DNA libraries - Enzyme purification - RNA isolation and purification - Isolation of poly-a+ mrna and synthesis of cdna - RT-PCR cloning - Isolation and purification of plasmid, and genomic DNA - Gene amplification, PCR and RT-PCR - Agarose and acrylamide gel electrophoresis, elution of DNA fragment from gel - Automated DNA sequencing on ABI 310 and ABI Competent cell preparation - Restriction mapping of DNA - Recombinant DNA cloning 2- Experience and lab skills in immunochemistry 00

12 - SDS-polyacrylamide gel electrophoresis - Western blotting - Radiolabeling of antibodies - Immunoblot assay - Radioimmunoassay - Protein purification including affinity chromatography and gel filtration - Recombinant protein production and purification techniques - Biotinylation of proteins 3- Cellular and other techniques - Different types of Tumor cell culturing and maintaining - Confocal microscopy, collagen degradation assay and quantification, transwell invasion assays, DNA microarray, RNA sequencing, RNA interference as well as working with in vivo models (i.e., animal handling and breeding). Teaching and sharing in teaching courses for post graduate students in the GEBRI: 1. Biology of Cancer 2. Antibodies I 3. Diagnostic Immnoassays. 4. Chemical and cellular immunology. 5. Medical Virology. 6. DNA Manipulations. 7. T-lymphocytes 8. Biochemistry of Nucelic Acid 9- Methods and applications of DNA markers Experience in the Quality Assurance programs and making the course specification of the following courses: 1. Biology of Cancer 2. Antibodies I 3. Diagnostic Immnoassays. 4. Chemical and cellular immunology LIST OF PUBLICATIONS 1. Sakr MA, Takino T, Domoto T, Nakano H, Wong RW, Sasaki M, Nakanuma Y, Sato H. GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. Cancer Sci. 2010, 10: AboElkhair M, Bazid AI, Sakr MA, Abd El-Razak AG, Sultan H (2012). Molecular characterization of fusion protein of Newcastle disease virus in Egypt (2012). Egyptian J Virol 9: ISSN:

13 3. Sakr MA., Gado MS and Elokil AA (2014). Molecular isolation, cloning and sequencing of chicken insulin-like growth factor 1 (IGF-1) gene from high producing exotic breed to produce transgenic chickens of native breeds in Egypt. 2 nd International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada, 8-12, April 2014, published as special issue on Annals of Agriculture Science, Moshtohor. ISSN: Isamail HA, Elsaid HH, Elfert AY, Sakr MA, Alnoamany WF. Evaluation of the Role of MicroRNA-21 in Regulating the Expression of Phosphatase and Tensin Homolog (PTEN) in Egyptian Hepatocellular Carcinoma Patients. Menoufia Medical Journal, 2015 (in press) Tarabees1 RZ, Hassanin ZH, Sakr MA, Zidan SM (2016). Molecular Screening of Some Virulence Factors Associated With Staphylococcus Aureus Isolated From Some Meat Products. Alex J. veterinary science, 48(1): ISSN: DOI: /ajvs Sakr M, Takino T, Sabit H, Nakada M, Li Z, and Sato H (2016). mir-150-5p and mir-133a suppress glioma cell proliferation and migration through targeting membrane-type-1 matrix metalloproteinase. Gene; 587 (2016) : Raiuf AA, Hamdy MM, Shalaan OF, Sakr MA, Abdel Rahman AA (2016). MTHFR A1298C polymorphism and the Vaso-Occlusive crisis in sickle cell disease. 1 st international conference of genetic engineering and biotechnology "Intergrative Science and Biotechnology" Sharm El-sheikh April Published as special issue in Research Journal of Applied Biotechnology (RJAB) ISSN: Shafiq A, Sakr M, Hemieda H, Elnasr M, Ismail H (2016). Association of MTHFR (C677T) and ACE (I/D) polymorphisms with hypertension and response to treatment among Egyptian. Egyptian J. of genetics and cytology (2016) 45(1): ISSN: X Shalaan O, Sakr M, Daif A, Elhalfawy K (2016). Rapid and simultaneous detection of β- thalassemia point mutations by reverse hybridization StripAssay 02

14 among Egyptian patients. Journal of Biochemistry, Microbiology, and Biotechnology (in press) vol. 4(1): ISSN: Training 1. Workshop about the "Bioanalyzer" held on Genetic Engineering and Biotechnology Research Institute, Sadat City, Minoufiya University, Egypt. 21 st November Workshop in the module entitled: (Proposal Writing Post Docs). Administrated by the DAAD Cairo held at Faculty of Veterinary Medicine, Sadat City, Minoufiya University, Egypt. 2 nd December Workshop and training course on (HPLC 1260) Adminstrated by Agilent Technologies Company held on Genetic Engineering and Biotechnology Research Institute, Sadat City, Minoufiya University, Egypt. 19 th December Trainee in the 2-day training workshop about PCR technique held in the Molecular Diagnostics andtheraputics Department, GEBRI, University of Sadat City from October Cairo Initiative (USAID) Alumni Workshop held on October 20th 2014 at Golden Tulip Hotel Flamenco located in 2 El Gezira El Wosta Street - Zamalek. Egypt 6. Workshop in "Ethical guidelines for Animal Care and Use in Scientific Reseaerch" organized by Institutional Animal Care & Use committee, Faculty of Veterinary Medicine, Sadat City held at Faculty of Veterinary Medicine, University of Sadat City, Egypt. 28 th November Workshop on " Nanotechnology and Applications" organized by Genetic Engineering and Biotechnology Research Institute (GEBRI) in cooperation with Naqaa Foundation of sceientific Reseaerch, Technology and Development, held at GEBRI, University of Sadat City, December Scientific and Professional Activities: Supervisor on MSc and PhD theses on different subjects of molecular diagnosis and molecular genetics. Partner and technical manager in a project titled Next generation sequencing to establish the Molecular Diagnostics, theraputice and genomics lab funded from the University of Sadat City and projects management section of the higher 04

15 minister of education, Egypt starting from April, Project code: LP SDAT Moustafa A. Sakr, and Hiroshi Sato. The role of membrane type 1- matrix metalloproteinase (MT1-MMP) in cancer invasion and metastasis. Attendance and oral presentation in the first Egypt Japan international Symposium of science and technology, June 8-10, Japan Attendance International symposium on tumor biology in Kanazawa, January 2007, Japan. Attendance International symposium on tumor biology in Kanazawa, January 2008, Japan. Attendance International symposium on tumor biology in Kanazawa, January 2009, Japan. Moustafa A. Sakr, and Hiroshi Sato. The interaction between GI24 and Membrane tupe1-matrix metalloproteinase (MT1-MMP) in cancer invasion and Metastasis. Attendence and poster presentation in the joint symposium of 5 th international symposium of institute network in Kanazawa June Japan. Attendance 19th Annual meeting of Japanese association for metastasis research. 17th June 2010, Japan. Attendance in International symposium on tumor biology in Kanazawa, cancer and host response, 14 th international congress on immunology satellite symposium th August 2010, Japan. Oral presentation about the scientific mission awrded by USAID in the GEBRI on 20 July 2014 titled by "MT1-MMP in cancer and tissue development" Giving a lecture titled by "how to study the gene function (molecular approach" 24 September Attendence in "International Symposium in Tumor Biology in Kanazawa. 8 April 2015,Takara hall, Kanazawa University hospital, Japan. Attendance in the "1 st Anuual National Conference for Expremintal Research". 5 th September 2015, children hospital Mansoura University. Attendance "The first Edition of Egyptian Biotech Joiners" organized by "Bio team Egypt" in collaboration with NRC on 29 th October

16 Moustafa Sakr (2016). mir-150-5p and mir-133a inhibit glioma cell proliferation and migration by targeting MMP14. Attendance and sharing with abstract (oral presentation) in the 1 st International Conference of Genetic Engineering and Biotechnology "Intergrative Science and Biotechnolohy" sharm El-sheikh April Organized by GEBRI, University of Sadat City. Member of the Egyptian society for biological sciences Referees: Prof. Hiroshi Sato The Head of Department of Molecular Virology and Oncology Cancer Research Institute, Kanazawa University Kakuma-machi, Kanazawa , Japan vhsato@kenroku.kanazawa-u.ac.jp Tel: Fax: Prof. Yoshio Endo Associate professor Central Research Resource Branch Cancer Research institute, Kanazawa University, Kakuma-machi, Kanazawa ,Japan yendo@kenroku.kanazawa-u.ac.jp Tel Stephen J. Weiss, M.D. E. Gifford and Love Barnett Upjohn Professor of Internal Medicine and Oncology Division of Molecular Medicine & Genetics Department of Internal Medicine Research Professor, Life Sciences Institute University of Michigan, USA. sjweiss@umich.edu Fax (1)